Journal: Oncology reports

Article Title: Knockdown of ubiquitin associated protein 2-like inhibits the growth and migration of prostate cancer cells.

doi: 10.3892/or.2014.3360

Figure Lengend Snippet: Figure 1. Expression analysis of UBAP2L in prostate carcinoma cells. (A) qRT-PCR analysis of the UBAP2L mRNA levels in the prostate cancer cell lines DU145, PC-3, LN-Cap and 22RV1. β-actin was used as an internal control. Data represent the mean ± SD of three independent experiments. (B) Western blot analysis of the UBAP2L protein levels in the prostate cancer cell lines DU145, PC-3, LN-Cap and 22RV1. GAPDH was used as a loading control. Data represent one out of three separate experiments.

Article Snippet: Proteins were probed overnight at 4 ̊C with the primary antibodies UBAP2L (1:2,000; Abcam; cat. ab70319) or gAPDH (1:50,000; Proteintech group, inc.; cat. 10494-1-AP), followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit igg (1:5,000; Santa Cruz; cat. sc-2054) or goat anti-mouse IgG (1:5,000; Santa Cruz; cat. sc-2005) at room temperature for 2 h. gAPDH was used as the internal standard.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot

Journal: Oncology reports

Article Title: Knockdown of ubiquitin associated protein 2-like inhibits the growth and migration of prostate cancer cells.

doi: 10.3892/or.2014.3360

Figure Lengend Snippet: Figure 3. UBAP2L expression in PC-3 and DU145 cells is inhibited by lentiviral-mediated shRNA. qRT-PCR analysis of the UBAP2L mRNA levels in (A) PC-3 and (C) DU145 cells after lentiviral infection. β-actin was used as an internal control. Data represent the mean ± SD of three independent experiments. **P<0.01, ***P<0.001, compared with Lv-shCon. Western blot analysis of the UBAP2L protein levels in (B) PC-3 and (D) DU145 cells after lentiviral infection. GAPDH was used as a loading control. Data represent one out of three separate experiments.

Article Snippet: Proteins were probed overnight at 4 ̊C with the primary antibodies UBAP2L (1:2,000; Abcam; cat. ab70319) or gAPDH (1:50,000; Proteintech group, inc.; cat. 10494-1-AP), followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit igg (1:5,000; Santa Cruz; cat. sc-2054) or goat anti-mouse IgG (1:5,000; Santa Cruz; cat. sc-2005) at room temperature for 2 h. gAPDH was used as the internal standard.

Techniques: Expressing, shRNA, Quantitative RT-PCR, Infection, Control, Western Blot